NSJ Bioreagents

PROX1 Antibody

Product Code:
 
NSJ-F47897
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the PROX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: HepG2 lysate; 2: mouse liver lysate; 3: SH-SY5Y lysate; Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
2 / 4
Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: SW620 lysate; 2: SH-SY5Y lysate
3 / 4
PROX1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
PROX1 antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue.
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Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: HepG2 lysate; 2: mouse liver lysate; 3: SH-SY5Y lysate; Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: SW620 lysate; 2: SH-SY5Y lysate
PROX1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
PROX1 antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue.

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CodeSizePrice
NSJ-F47897-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47897-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PROX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Prospero homeobox protein 1 is a transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 492-520 from the human protein was used as the immunogen for this PROX1 antibody.
Limitation:
This PROX1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q92786