NSJ Bioreagents

HIF-1 alpha Antibody

Product Code:
 
NSJ-F50744
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the HIF-1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot analysis of HIF-1 alpha antibody and MDA-MB231 lysate
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Western blot analysis of HIF-1 alpha antibody and mouse cerebellum tissue lysate
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Confocal immunofluorescent analysis of HIF-1 alpha antibody with MDA-MB231 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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IHC analysis of FFPE human hepatocarcinoma tissue stained with HIF-1 alpha antibody
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Western blot analysis of HIF-1 alpha antibody and MDA-MB231 lysate
Western blot analysis of HIF-1 alpha antibody and mouse cerebellum tissue lysate
Confocal immunofluorescent analysis of HIF-1 alpha antibody with MDA-MB231 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human hepatocarcinoma tissue stained with HIF-1 alpha antibody

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CodeSizePrice
NSJ-F50744-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50744-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 728-757 from the human protein was used as the immunogen for this HIF-1 alpha antibody.
Limitation:
This HIF-1 alpha antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q16665