NSJ Bioreagents

DLL3 Antibody

Product Code:
 
NSJ-F51660
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the DLL3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 9
Confocal immunofluorescent analysis of DLL3 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
2 / 9
Western blot analysis of DLL3 antibody and A375 lysate. Predicted molecular weight: ~65 kDa.
3 / 9
IHC analysis of FFPE human brain tissue stained with DLL3 antibody
4 / 9
Immunofluorescent staining of human U-2 OS cells with DLL3 antibody (green), Phalloidin (red) and DAPI nuclear stain (blue).
5 / 9
Immunofluorescent staining of human U-2 OS cells with DLL3 antibody (green) and Phalloidin (red).
6 / 9
Western blot testing of human 1) A375, 2) U-251 MG, 3) NCI-H1299, 4) brain, 5) mouse brain and 6) rat brain lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.
7 / 9
Western blot testing of human 1) NCI-H1299, 2) brain, 3) mouse brain and 4) rat brain lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.
8 / 9
IHC staining of FFPE human testis with DLL3 antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.
9 / 9
IHC staining of FFPE human brain with DLL3 antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.
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Confocal immunofluorescent analysis of DLL3 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Western blot analysis of DLL3 antibody and A375 lysate. Predicted molecular weight: ~65 kDa.
IHC analysis of FFPE human brain tissue stained with DLL3 antibody
Immunofluorescent staining of human U-2 OS cells with DLL3 antibody (green), Phalloidin (red) and DAPI nuclear stain (blue).
Immunofluorescent staining of human U-2 OS cells with DLL3 antibody (green) and Phalloidin (red).
Western blot testing of human 1) A375, 2) U-251 MG, 3) NCI-H1299, 4) brain, 5) mouse brain and 6) rat brain lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.
Western blot testing of human 1) NCI-H1299, 2) brain, 3) mouse brain and 4) rat brain lysate with DLL3 antibody. Predicted molecular weight: ~65 kDa.
IHC staining of FFPE human testis with DLL3 antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain with DLL3 antibody. HIER: boil tissue sections in pH 9 EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-F51660-0.05ML0.05 ml£260.00
Quantity:
NSJ-F51660-0.2ML0.2 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the DLL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity). [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 519-548 from the human protein was used as the immunogen for this DLL3 antibody.
Limitation:
This DLL3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9NYJ7