NSJ Bioreagents

PROX1 Antibody

Product Code:
 
NSJ-F47894
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the PROX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Fluorescent confocal image of A2058 cell stained with PROX1 antibody at 1:25. PROX1 immunoreactivity is localized to the nucleus.
2 / 4
Western blot analysis of lysate from (1) human A2058 and (2) rat PC-12 cell line using PROX1 antibody at 1:1000. Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
3 / 4
PROX1 antibody western blot analysis in A2058 lysate
4 / 4
Western blot analysis of PROX1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).
❮❯

Fluorescent confocal image of A2058 cell stained with PROX1 antibody at 1:25. PROX1 immunoreactivity is localized to the nucleus.
Western blot analysis of lysate from (1) human A2058 and (2) rat PC-12 cell line using PROX1 antibody at 1:1000. Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
PROX1 antibody western blot analysis in A2058 lysate
Western blot analysis of PROX1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).

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CodeSizePrice
NSJ-F47894-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47894-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the PROX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The expression pattern of Prox1 suggests that it has a role in a variety of embryonic tissues, including lens. Prox mRNA is present in many different human tissues with lens demonstrating the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 492-522 from the human protein was used as the immunogen for this PROX1 antibody.
Limitation:
This PROX1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Rat
Species Reactivity (Predicted):
Mouse
Uniprot #:
Q92786