NSJ Bioreagents

Cdk4 Antibody

Product Code:
 
NSJ-F52368
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Storage:
 
Aliquot the Cdk4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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IHC testing of paraffin-embedded rat small intestine using Cdk4 antibody at 1:25.
2 / 3
IHC analysis of FFPE rat brain section using Cdk4 antibody at 1:25.
3 / 3
Fluorescent image of MCF-7 cells stained with Cdk4 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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IHC testing of paraffin-embedded rat small intestine using Cdk4 antibody at 1:25.
IHC analysis of FFPE rat brain section using Cdk4 antibody at 1:25.
Fluorescent image of MCF-7 cells stained with Cdk4 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

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CodeSizePrice
NSJ-F52368-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52368-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
IHC (Paraffin): 1:25,Immunofluorescence: 1:25
Application Note:
Titration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 272-303 from the rat protein was used as the immunogen for this Cdk4 antibody.
Limitation:
This Cdk4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P35426