NSJ Bioreagents

DHFR Antibody

Product Code:
 
NSJ-F50355
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the DHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of DHFR antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the DHFR gene. Predicted molecular weight ~22 kDa.
2 / 3
DHFR antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma.
3 / 3
Western blot analysis of DHFR antibody and mouse liver tissue lysate
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Western blot analysis of DHFR antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the DHFR gene. Predicted molecular weight ~22 kDa.
DHFR antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma.
Western blot analysis of DHFR antibody and mouse liver tissue lysate

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CodeSizePrice
NSJ-F50355-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50355-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 135-164 from mouse Dihydrofolate reductase was used as the immunogen for this DHFR antibody.
Limitation:
This DHFR antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Mouse
Uniprot #:
P00375