NSJ Bioreagents

Shh Antibody / Sonic Hedgehog

Product Code:
 
NSJ-F53217
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot the Shh antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of Shh antibody at 1:2000 dilution + mouse stomach lysate; Predicted molecular weight: 45/27/19 kDa (1)~
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Western blot testing of Shh antibody at 1:2000 dilution. Lane 1: F9 lysate; 2: mouse stomach lysate; 3: NIH3T
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Western blot testing of Shh antibody at 1:2000 dilution + mouse stomach lysate; Predicted molecular weight: 45/27/19 kDa (1)~
Western blot testing of Shh antibody at 1:2000 dilution. Lane 1: F9 lysate; 2: mouse stomach lysate; 3: NIH3T

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CodeSizePrice
NSJ-F53217-0.08ML0.08 ml£260.00
Quantity:
NSJ-F53217-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000-2000
Application Note:
Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.

Description:
Sonic hedgehog intercellular signaling is essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This mouse Shh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 58-91 amino acids from the N-terminal region of mouse Sonic hedgehog.
Limitation:
This Shh antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q62226