NSJ Bioreagents

Pou5f1 Antibody

Product Code:
 
NSJ-F52574
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Zebrafish
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot the Pou5f1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot analysis of lysate from zebrafish tissue lysate using Pou5f1 antibody at 1:1000.
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Western blot analysis of lysate from zebrafish tissue lysate using Pou5f1 antibody at 1:1000.

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CodeSizePrice
NSJ-F52574-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52574-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the Pou5f1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This Pou5f1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 128-161 amino acids from the N-terminal region of zebrafish Pou5f1.
Limitation:
This Pou5f1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Zebrafish
Uniprot #:
Q90270