NSJ Bioreagents

His Tag Antibody

Product Code:
 
NSJ-F52070
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot the His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 2
Western blot testing of 1) 293T/17 cell lysate, 2) 12-tag transfected 293T/17 cell lysate and 3) 12-tag recombinant protein with His Tag antibody.~
2 / 2
Western blot analysis of His Tag antibody and a His tagged protein.
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Western blot testing of 1) 293T/17 cell lysate, 2) 12-tag transfected 293T/17 cell lysate and 3) 12-tag recombinant protein with His Tag antibody.~
Western blot analysis of His Tag antibody and a His tagged protein.

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CodeSizePrice
NSJ-F52070-0.05ML0.05 ml£260.00
Quantity:
NSJ-F52070-0.2ML0.2 ml£502.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Poly-His peptide was used as the immungen for the His Tag antibody.
Limitation:
This His Tag antibody is available for research use only.
Purity:
Purified