NSJ Bioreagents

NANOG Antibody

Product Code:
 
NSJ-F44248
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Storage:
 
Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC analysis of FFPE human testis tissue stained with NANOG antibody
2 / 3
Immunofluorescence analysis of NANOG antibody with HeLa cells . Primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
3 / 3
Flow cytometric analysis of HeLa cells using NANOG antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC analysis of FFPE human testis tissue stained with NANOG antibody
Immunofluorescence analysis of NANOG antibody with HeLa cells . Primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Flow cytometric analysis of HeLa cells using NANOG antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F44248-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44248-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. It is a transcription factor in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG is thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. [Wiki]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 94-123 from the human protein was used as the immunogen for this NANOG antibody.
Limitation:
This NANOG antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Primate
Uniprot #:
Q9H9S0