NSJ Bioreagents

TRIM28 Antibody / KAP1

Product Code:
 
NSJ-F54620
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the KAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.
2 / 4
Western blot testing of human K562 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with KAP1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
IHC testing of FFPE human testis tissue with KAP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.
Western blot testing of human K562 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with KAP1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human testis tissue with KAP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54620-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54620-0.4ML0.4 ml£502.00
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Further Information

Application Details :
Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the KAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 178-207 from the human protein was used as the immunogen for the KAP1 antibody.
Limitation:
This KAP1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q13263