NSJ Bioreagents

COMP Antibody / TSP5

Product Code:
 
NSJ-F54435
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the TSP5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human prostate carcinoma tissue with TSP5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) human skeletal muscle, 2) mouse ATDC5, 3) HUVEC, 4) human Jurkat, 5) human MG-63 and 6) human U-2 OS lysate with TSP5 antibody. Expected molecular weight: 83-100 kDa depending on glycosylation level.
3 / 4
Western blot testing of rat skeletal muscle lysate with TSP5 antibody. Expected molecular weight: 83-100 kDa depending on glycosylation level.
4 / 4
Flow cytometry testing of fixed and permeabilized human HepG2 cells with TSP5 antibody; Blue=isotype control, Green= TSP5 antibody.
❮❯

IHC testing of FFPE human prostate carcinoma tissue with TSP5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human skeletal muscle, 2) mouse ATDC5, 3) HUVEC, 4) human Jurkat, 5) human MG-63 and 6) human U-2 OS lysate with TSP5 antibody. Expected molecular weight: 83-100 kDa depending on glycosylation level.
Western blot testing of rat skeletal muscle lysate with TSP5 antibody. Expected molecular weight: 83-100 kDa depending on glycosylation level.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with TSP5 antibody; Blue=isotype control, Green= TSP5 antibody.

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CodeSizePrice
NSJ-F54435-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54435-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the TSP5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
COMP/TSP5 is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 314-343 from the human protein was used as the immunogen for the TSP5 antibody.
Limitation:
This TSP5 antibody is available for research use only.
Localization:
Secreted
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P49747