NSJ Bioreagents

TBX1 Antibody

Product Code:
 
NSJ-F54472
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the TBX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of human HUVEC with TBX1 antibody. Predicted molecular weight ~43 kD.
2 / 5
Western blot testing of human HLVEC with TBX1 antibody. Predicted molecular weight ~43 kD.
3 / 5
Immunofluorescent staining of fixed and permeabilized human A2058 cells with TBX1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
IHC testing of FFPE human breast tissue with TBX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
IHC testing of FFPE human testis tissue with TBX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Western blot testing of human HUVEC with TBX1 antibody. Predicted molecular weight ~43 kD.
Western blot testing of human HLVEC with TBX1 antibody. Predicted molecular weight ~43 kD.
Immunofluorescent staining of fixed and permeabilized human A2058 cells with TBX1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human breast tissue with TBX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human testis tissue with TBX1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54472-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54472-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the TBX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 327-356 from the human protein was used as the immunogen for the TBX1 antibody.
Limitation:
This TBX1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O43435