NSJ Bioreagents

ETHE1 Antibody

Product Code:
 
NSJ-F54868
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the ETHE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human colon carcinoma tissue with ETHE1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of mouse kidney tissue lysate with ETHE1 antibody. Predicted molecular weight ~28 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ETHE1 antibody.
4 / 4
Flow cytometry testing of human HEK293 cells with ETHE1 antibody; Blue=isotype control, Green= ETHE1 antibody.
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IHC testing of FFPE human colon carcinoma tissue with ETHE1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse kidney tissue lysate with ETHE1 antibody. Predicted molecular weight ~28 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ETHE1 antibody.
Flow cytometry testing of human HEK293 cells with ETHE1 antibody; Blue=isotype control, Green= ETHE1 antibody.

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CodeSizePrice
NSJ-F54868-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54868-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the ETHE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ETHE1 is a sulfur dioxygenase that localizes within the mitochondrial matrix. The enzyme functions in sulfide catabolism. Mutations in its gene result in ethylmalonic encephalopathy.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 103-130 from the human protein was used as the immunogen for the ETHE1 antibody.
Limitation:
This ETHE1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
O95571