NSJ Bioreagents

LC3B Antibody / MAP1LC3B

Product Code:
 
NSJ-F54364
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the MAP1LC3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human brain tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human liver tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human HepG2 cell lysate with MAP1LC3B antibody. Predicted molecular weight ~15 kDa.
4 / 5
Western blot testing of mouse NIH 3T3 cell lysate with MAP1LC3B antibody. Predicted molecular weight ~15 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human HepG2 cells with MAP1LC3B antibody; Blue=isotype control, Green= MAP1LC3B antibody.
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IHC testing of FFPE human brain tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human HepG2 cell lysate with MAP1LC3B antibody. Predicted molecular weight ~15 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with MAP1LC3B antibody. Predicted molecular weight ~15 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with MAP1LC3B antibody; Blue=isotype control, Green= MAP1LC3B antibody.

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CodeSizePrice
NSJ-F54364-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54364-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the MAP1LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 9-33 from the human protein was used as the immunogen for the MAP1LC3B antibody.
Limitation:
This MAP1LC3B antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9GZQ8