NSJ Bioreagents

MLF1 Antibody

Product Code:
 
NSJ-F54428
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the MLF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of human MCF7 lysate with MLF1 antibody. Predicted molecular weight ~31 kDa.
2 / 5
Western blot testing of mouse heart lysate with MLF1 antibody. Predicted molecular weight ~31 kDa.
3 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with MLF1 antibody.
4 / 5
IHC testing of FFPE human testis tissue with MLF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human CCRF-CEM cells with MLF1 antibody; Blue=isotype control, Green= MLF1 antibody.
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Western blot testing of human MCF7 lysate with MLF1 antibody. Predicted molecular weight ~31 kDa.
Western blot testing of mouse heart lysate with MLF1 antibody. Predicted molecular weight ~31 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with MLF1 antibody.
IHC testing of FFPE human testis tissue with MLF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human CCRF-CEM cells with MLF1 antibody; Blue=isotype control, Green= MLF1 antibody.

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CodeSizePrice
NSJ-F54428-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54428-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the MLF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 215-244 from the human protein was used as the immunogen for the MLF1 antibody.
Limitation:
This MLF1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P58340