NSJ Bioreagents

GPA33 Antibody / Cell surface A33 antigen

Product Code:
 
NSJ-F55008
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the GPA33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) 293T and 2) HT-29 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.
2 / 4
Western blot testing of human 1) K562, 2) HEK293 and 3) MDA-MB-231 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.
3 / 4
IHC testing of FFPE human colon carcinoma tissue with GPA33 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human MDA-MB-231 cells with GPA33 antibody; Blue=isotype control, Green= GPA33 antibody.
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Western blot testing of human 1) 293T and 2) HT-29 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.
Western blot testing of human 1) K562, 2) HEK293 and 3) MDA-MB-231 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.
IHC testing of FFPE human colon carcinoma tissue with GPA33 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-231 cells with GPA33 antibody; Blue=isotype control, Green= GPA33 antibody.

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CodeSizePrice
NSJ-F55008-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55008-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
GPA33 is a cell surface antigen that is expressed in greater than 95% of human colon cancers. It may play a role in cell-cell recognition and signaling.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 264-293 from the human protein was used as the immunogen for the GPA33 antibody.
Limitation:
This GPA33 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q99795