NSJ Bioreagents

Beta Glucuronidase Antibody / Gusb

Product Code:
 
NSJ-F55031
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Beta Glucuronidase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human liver tissue with Beta Glucuronidase antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human colon carcinoma tissue with Beta Glucuronidase antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) HT-29, 2) WiDr, 3) K562, 4) A2058, 5) HL60, 6) mouse liver and 7) mouse lung lysate with Beta Glucuronidase antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.
4 / 4
Flow cytometry testing of human WiDr cells with Beta Glucuronidase antibody; Blue=isotype control, Green= Beta Glucuronidase antibody.
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IHC testing of FFPE human liver tissue with Beta Glucuronidase antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon carcinoma tissue with Beta Glucuronidase antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HT-29, 2) WiDr, 3) K562, 4) A2058, 5) HL60, 6) mouse liver and 7) mouse lung lysate with Beta Glucuronidase antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.
Flow cytometry testing of human WiDr cells with Beta Glucuronidase antibody; Blue=isotype control, Green= Beta Glucuronidase antibody.

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CodeSizePrice
NSJ-F55031-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55031-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:2000-1:4000
Description:
The GUSB gene encodes beta-glucuronidase, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987). The GUSB gene is mutated in mucopolysaccharidosis type VII.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 335-362 from the human protein was used as the immunogen for the Beta Glucuronidase antibody.
Limitation:
This Beta Glucuronidase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P08236