NSJ Bioreagents

CDH20 Antibody / Cadherin 20

Product Code:
 
NSJ-F55047
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Cadherin 20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human HepG2 cell lysate with Cadherin 20 antibody. Predicted molecular weight ~89 kDa.
2 / 4
Western blot testing of 1) human brain and 2) mouse brain tissue lysate with Cadherin 20 antibody. Predicted molecular weight ~89 kDa.
3 / 4
IHC testing of FFPE human brain tissue with Cadherin 20 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HepG2 cells with Cadherin 20 antibody; Blue=isotype control, Green= Cadherin 20 antibody.
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Western blot testing of human HepG2 cell lysate with Cadherin 20 antibody. Predicted molecular weight ~89 kDa.
Western blot testing of 1) human brain and 2) mouse brain tissue lysate with Cadherin 20 antibody. Predicted molecular weight ~89 kDa.
IHC testing of FFPE human brain tissue with Cadherin 20 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with Cadherin 20 antibody; Blue=isotype control, Green= Cadherin 20 antibody.

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CodeSizePrice
NSJ-F55047-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55047-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 111-140 from the human protein was used as the immunogen for the Cadherin 20 antibody.
Limitation:
This Cadherin 20 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9HBT6