NSJ Bioreagents

BRAF Antibody

Product Code:
 
NSJ-RQ5438
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CCG-2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the BRAF antibody at -20oC.
1 / 2
IHC staining of FFPE human ovarian cancer with BRAF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of human 1) brain and 2) HepG2 lysate with BRAF antibody. Predicted molecular weight: 85-95 kDa.
❮❯

IHC staining of FFPE human ovarian cancer with BRAF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 1) brain and 2) HepG2 lysate with BRAF antibody. Predicted molecular weight: 85-95 kDa.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ5438-100ul100 ul£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the BRAF antibody should be determined by the researcher.
Description:
The BRAF gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human B Raf was used as the immunogen for the BRAF antibody.
Limitation:
This BRAF antibody is available for research use only.
Localization:
Cytoplasmic, nuclear, cell membrane
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P15056