NSJ Bioreagents

Histone H2AX Antibody / H2AFX

Product Code:
 
NSJ-RQ5464
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BIA-8
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the H2AFX antibody at -20oC.
1 / 2
IHC staining of FFPE rat lung tissue with H2AFX antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of human Raji cell lysate with H2AFX antibody. Predicted molecular weight ~15 kDa.
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IHC staining of FFPE rat lung tissue with H2AFX antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate with H2AFX antibody. Predicted molecular weight ~15 kDa.

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NSJ-RQ5464-100ul100 ul£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry: 1:50-1:200
Application Note:
Optimal dilution of the H2AFX antibody should be determined by the researcher.
Description:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Histone H2A.X / H2AFX was used as the immunogen for the H2AFX antibody.
Limitation:
This H2AFX antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P16104