NSJ Bioreagents

CD19 Antibody / B-lymphocyte marker

Product Code:
 
NSJ-RQ5485
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AOFO-3
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CD19 antibody at -20oC.
1 / 7
Western blot testing of human Ramos cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
2 / 7
Western blot testing of human Ramos cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
3 / 7
Western blot testing of human 1) Raji and 2) Daudi cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
4 / 7
IHC staining of FFPE human Hodgkin's lymphoma tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human esophageal carcinoma tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE mouse skin tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE rat stomach tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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Western blot testing of human Ramos cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
Western blot testing of human Ramos cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
Western blot testing of human 1) Raji and 2) Daudi cell lysate with CD19 antibody. Expected molecular weight: 60-100 kDa depending on glycosylation level.
IHC staining of FFPE human Hodgkin's lymphoma tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal carcinoma tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse skin tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat stomach tissue with CD19 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

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NSJ-RQ5485-100ul100 ul£535.00
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Further Information

Application Details:
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the CD19 antibody should be determined by the researcher.
Description:
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human CD19 was used as the immunogen for the CD19 antibody.
Limitation:
This CD19 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P15391