NSJ Bioreagents

MPI Antibody / Mannose Phosphate Isomerase

Product Code:
 
NSJ-RQ5624
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
11I4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MPI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human U-2 OS cells with MPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPI antibody.
2 / 2
Western blot testing of human 1) Caco-2 and 2) HeLa lysate with MPI antibody. Predicted molecular weight ~47 kDa.
❮❯

Flow cytometry testing of human U-2 OS cells with MPI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MPI antibody.
Western blot testing of human 1) Caco-2 and 2) HeLa lysate with MPI antibody. Predicted molecular weight ~47 kDa.

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NSJ-RQ5624-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MPI antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids A2-K99) was used as the immunogen for the MPI antibody.
Limitation:
This MPI antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P34949