NSJ Bioreagents

Chromogranin A Antibody

Product Code:
 
NSJ-RQ6461
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the Chromogranin A antibody can be stored for up to one month at 4oC. For long-term aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
Flow cytometry testing of mouse HEPA1-6 cells with Chromogranin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Chromogranin A antibody.
2 / 4
Western blot testing of mouse 1) stomach and 2) Neuro-2a cell lysate with Chromogranin A antibody. Expected molecular weight: 50-75 kDa depending on glycosylation level.
3 / 4
IHC staining of FFPE rat colon tissue with Chromogranin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE rat colon tissue with Chromogranin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of mouse HEPA1-6 cells with Chromogranin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Chromogranin A antibody.
Western blot testing of mouse 1) stomach and 2) Neuro-2a cell lysate with Chromogranin A antibody. Expected molecular weight: 50-75 kDa depending on glycosylation level.
IHC staining of FFPE rat colon tissue with Chromogranin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with Chromogranin A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-RQ6461-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Chromogranin A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CHGA, also known as CGA or chromogranin A, is an acidic protein costored and coreleased with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An E. coli-derived mouse protein (amino acids D378-K425) was used as the immunogen for the Chromogranin A antibody.
Limitation:
This Chromogranin A antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse
Uniprot #:
P26339