NSJ Bioreagents

EHD2 Antibody

Product Code:
 
NSJ-RQ6476
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the EHD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 2
Immunofluorescent staining of FFPE human A549 cells with EHD2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 2
Western blot testing of human U-87 MG cell lysate with EHD2 antibody. Predicted molecular weight ~61 kDa.
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Immunofluorescent staining of FFPE human A549 cells with EHD2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human U-87 MG cell lysate with EHD2 antibody. Predicted molecular weight ~61 kDa.

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NSJ-RQ6476-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the EHD2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
EH-domain containing 2, also known as EHD2, is a human gene belonging to the EHD protein family. This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KKQLILKLPVIFAKIQLEHH from the human protein were used as the immunogen for the EHD2 antibody.
Limitation:
This EHD2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q9NZN4