ProSci

CD80 Antibody [IDEC-114 (Galiximab)], Rabbit IgG

Product Code:
 
PSI-24-500
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG lambda
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
IDEC-114 (Galiximab)
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Blocking
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Modulate
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody IDEC-114 (Galixim736) </b> Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of IDEC-114 (Galixim00) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-500- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.
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<b> Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody IDEC-114 (Galixim736) </b> Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of IDEC-114 (Galixim00) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-500- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

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PSI-24-500-0.2mg0.2mg£681.00
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Further Information

Additional Names:
B7-1, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)
NCBI Gene ID #:
941
NCBI Official Name:
CD80 molecule
NCBI Official Symbol:
CD80
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Other, Immunology
Swissprot #:
P33681
User NOte:
For research use only.