ProSci

TDRD3 Antibody [RAB-C419], Rabbit IgG

Product Code:
 
PSI-24-858
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RAB-C419
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunoprecipitation-Mass Spectrometry (IP-MS)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Flow cytometry using the Anti-TDRD3 antibody R </b> Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of R24-858- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of HeLa cells with anti-TDRD3 (R/b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of R24-858) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing nucleoli- nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-858- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-TDRD3 antibody R </b> Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of R24-858- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of HeLa cells with anti-TDRD3 (R/b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of R24-858) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing nucleoli- nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-858- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

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CodeSizePrice
PSI-24-858-0.2mg0.2mg£681.00
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Further Information

Additional Names:
Tudor domain-containing protein 3, anti-TDRD3-RAB-C419, TDRD3-IS1-73I
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was obtained by recombinant antibody (rAb) phage display recognizing TDRD3 protein under non-denaturing conditions.
NCBI Gene ID #:
81550
NCBI Official Name:
tudor domain containing 3
NCBI Official Symbol:
TDRD3
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Cancer, Cell Cycle
Swissprot #:
Q9H7E2
User NOte:
For research use only.