ProSci

FOXP4 Antibody [RAB-S40], Rabbit IgG

Product Code:
 
PSI-24-862
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RAB-S40
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Spiked IP
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Flow cytometry using the anti-FOXP4 antibody Rb01717). </b> HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of R4-862- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Western Blot using anti-FOXP4 antibody Rb01717) </b> Nuclear lysate of K562 cells (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of R4-862) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Flow cytometry using the anti-FOXP4 antibody Rb01717). </b> HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of R4-862- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Western Blot using anti-FOXP4 antibody Rb01717) </b> Nuclear lysate of K562 cells (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of R4-862) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

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PSI-24-862-0.2mg0.2mg£681.00
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Further Information

Additional Names:
Fork head-related protein-like A, Forkhead box protein P4, anti-FOXP4-RAB-S40, S0014_H01-HR6897B.001_D07
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP4 protein under non-denaturing conditions.
NCBI Gene ID #:
116113
NCBI Official Name:
forkhead box P4
NCBI Official Symbol:
FOXP4
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Cancer, Cell Cycle
Swissprot #:
Q8IVH2
User NOte:
For research use only.