ProSci

RRM2 Antibody [SAIC-30C-18], Rabbit IgG

Product Code:
 
PSI-24-328
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SAIC-30C-18
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immuno-MRM
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Western Blot using anti-RRM2 antibody SAIC-30C-18 ( </b> Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-30C-18 (24-328) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Immunofluorescence staining of HeLa cells with anti-RRM2 (SAIC-30C-18 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-30C-18 (24-328) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-328- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Western Blot using anti-RRM2 antibody SAIC-30C-18 ( </b> Jurkat cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-30C-18 (24-328) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Immunofluorescence staining of HeLa cells with anti-RRM2 (SAIC-30C-18 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-30C-18 (24-328) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-328- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

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PSI-24-328-0.2mg0.2mg£681.00
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Further Information

Additional Names:
RR2, R2, RR2M, ribonucleotide reductase regulatory subunit M2, Ribonucleoside-diphosphate reductase subunit M2
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The KLH-conjugated peptide "IEQEFLTEALPVK" derived from Ribonucleoside-diphosphate reductase subunit M2 (RRM2). RRM2 provides precursors required for DNA synthesis.
NCBI Gene ID #:
6241
NCBI Official Name:
ribonucleotide reductase regulatory subunit M2
NCBI Official Symbol:
RRM2
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
P31350
User NOte:
For research use only.