ProSci

beta-NGF Antibody (biotin)

Product Code:
 
PSI-38-336
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-38-336-0.025mg0.025mg£505.00
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This product comes from: United States.
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Further Information

Additional Names:
NGF-beta, beta-Nerve Growth Factor
Application Note:
Sandwich ELISA: To detect Humanbeta-NGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Humanbeta-NGF as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Humanbeta-NGF.
Western Blot
To detect Humanbeta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Humanbeta-NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
beta-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. beta-NGF is a potent neurotrophic factor that signals through its receptorbeta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. beta-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. The functional form of Recombinant Humanbeta-NGF is a non-covalently-linked homodimer of two 13.5 kDa, polypeptide monomers that each contain 120 amino acids and three disulfide bonds, which are required for biological activity.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure recombinant Human beta-NGF. Anti-Human beta-NGF specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
4803
NCBI Official Name:
nerve growth factor
NCBI Official Symbol:
NGF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01138
Protein GI Number:
P01138.3
Purification:
beta-NGF specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Immunology,Obesity,Neuroscience,Stem Cell,Other
Swissprot #:
P01138
User NOte:
Centrifuge vial prior to opening.