ProSci

MIP-5 (CCL15) Antibody (biotin)

Product Code:
 
PSI-38-338
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect hMIP-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hMIP-5.
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To detect hMIP-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hMIP-5.

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CodeSizePrice
PSI-38-338-0.025mg0.025mg£505.00
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Further Information

Additional Names:
Macrophage Inflammatory Protein-5, CCL15, HCC-2, Lkn-1
Application Note:
Sandwich ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hMIP-5.
Western Blot
To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
MIP-5 is a CC chemokine that is expressed in the heart, skeletal muscle and adrenal gland. MIP-5 primarily signals through the CCR1 receptor, but has also been found to bind to CCR3. MIP-5 is chemotactic towards T cells and monocytes. Recombinant Human MIP-5 is a 10.1 kDa protein containing 92 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-5. Anti-Human MIP-5 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
6359
NCBI Official Name:
C-C motif chemokine ligand 15
NCBI Official Symbol:
CCL15
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q16663
Protein GI Number:
Q16663.3
Purification:
MIP-5 specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Immunology
Swissprot #:
Q16663
User NOte:
Centrifuge vial prior to opening.