Further Information
Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF
p35: Interleukin-12 subunit alpha, IL-12 subunit p35, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35 kDa subunit (CLMF p35), NK cell Stimulating Factor Chain 1
p40: Interleukin-12 subunit beta, IL-12 subunit p40, IL-12B, CytotoxicLymphocyte Maturation Factor 40 kDa subunit (CLMF p40), NK cell Stimulating Factor Chain 2
Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-12 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hIL-12.
Western Blot
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
batch dependent
Biotin
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-12. Anti-Human IL-12 specific antibody was purified by affinity chromatography and then biotinylated.
p35: 3592 p40: 3593
interleukin 12A, interleukin 12B
IL12A , IL12B
Homo sapiens
Lyophilized
p35: P29459 p40: P29460
P29459.2, P29460.1
IL-12 specific antibody was purified by affinity chromatography and then biotinylated
COVID-19,Immunology,Cancer,Stem Cell,Other
p35: P29459 p40: P29460
Centrifuge vial prior to opening.