ProSci

sFas Receptor Antibody (biotin)

Product Code:
 
PSI-38-347
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect Human sFas Receptor by sandwich ELISA (using 100 ml/well) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti- Human sFas Receptor as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human sFas Receptor.
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To detect Human sFas Receptor by sandwich ELISA (using 100 ml/well) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti- Human sFas Receptor as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human sFas Receptor.

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CodeSizePrice
PSI-38-347-0.025mg0.025mg£505.00
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Further Information

Additional Names:
soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen
Application Note:
Sandwich ELISA: To detect Human sFas Receptor by sandwich ELISA (using 100 ml/well) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti- Human sFas Receptor as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human sFas Receptor.
Western Blot
To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human sFas Receptor is 1.5 ? 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Fas and Fas Ligand (FasL) belong to the TNF superfamily, and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and a 145 amino acid cytoplasmic domain. Recombinant Human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR-homologous cysteine-rich extracellular Fas domain.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human sFas Receptor. Anti-Human sFas Receptor specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
355
NCBI Official Name:
Fas cell surface death receptor
NCBI Official Symbol:
FAS
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P25445
Protein GI Number:
P25445.1
Purification:
sFas Receptor specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Infectious Disease,Other,Cancer
Swissprot #:
P25445
User NOte:
Centrifuge vial prior to opening.