Anti-RAGE (Human) mAb
Product Code:
MBL-CY-M1038
MBL-CY-M1038
Host Type:
Mouse
Mouse
Antibody Isotype:
IgG1
IgG1
Antibody Clonality:
Monoclonal
Monoclonal
Antibody Clone:
YK-2B4
YK-2B4
Regulatory Status:
RUO
RUO
Target Species:
Human
Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Western Blot (WB)
Shipping:
4°C
4°C
Storage:
-20°C
-20°C
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
MBL-CY-M1038 | 100 ug | £512.00 |
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
- Further Information
- Documents
- References
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Further Information
Applications:
WB - 0.5-1 ug/mL
Background:
RAGE is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules that is expressed in a variety of cell lines, including end othelial cells, smooth muscle cells, mononuclear phagocytes, pericytes, neurons, cardiac myocytes, mesangial cells and hepatocytes (1, 2). RAGE interacts with different structures to transmit a signal into the cell and recognizes three-dimensional structures rather than specific amino acid sequences. Therefore, RAGE seems to fulfill the requirements of a pattern-recognition receptor. As a member of the immunoglobulin superfamily, it interacts with a diverse class of ligands, including AGEs (3, 4), HMGB1 (also known as Amphoterin) (5), amyloid β-peptide (6), amyloid A (7), leukocyte adhesion receptors (8), prions (9), Escherichia coli curli operons (10), β-sheet fibrils (11) and several members of the S100 protein superfamily including S100/calgranulins (12). Thus RAGE may have potential involvement in several pathological processes including inflammation, diabetes, Alzheimer?s disease (AD), systemic amyloidosis, and tumor growth (13). It has been reported that soluble RAGE (sRAGE) isoforms that could act as decoy receptors may be cleaved proteolytically from the native RAGE expressed on the cell surface (14), as well as endogenous secretory RAGE (esRAGE) derived from a splice variant, carrying all of the extracellular domains. This proteolytic generation of sRAGE was initially descr ibed as occurring in mice (15). These diverse types of sRAGE in human plasma could exert significant protective effects against RAGE-mediated toxicity.
Concentration:
1.0 ug/uL
Formulation:
20 mM phosphate buffer (pH 7.5), 300 mM NaCl, 50 % glycerol.
Immunogen Translated:
Extracellular domain of Recombinant human RAGE
MW:
46-54 kDa
Shelf Life:
1 year
Source:
The antibody is produced by immunizing mice with a recombinant extacellular domain of human RAGE. IgG is purified by protein A-Sepharose chromatography.
Target:
RAGE
Documents
References
1. Neeper M, Schmidt AM, Brett J et al.. J Biol Che
m 1992, 267: 14998-5004.
2. Brett J, Schmidt AM, Yan SD et al. Am J Pathol 1
993, 143: 1699-712.
3. Neeper M, Schmidt AM, Brett J, et al. J Biol Che
m 1992, 267: 14998-15004.
4. Schmidt AM, Vianna M, Gerlach M, et al. J Biol C
hem 1992, 267: 14987-14997
5. Hori O, Brett J, Slattery T, Cao R et al. J Biol
Chem 1995, 270: 25752-25761.
6. Yan SD, Zhu H, Fu J, Yan SF, Roher A et al. Proc
Natl Acad Sci U S A 1997, 94: 5296-5301.
7. Yan SD, Zhu H, Zhu A, Golabek A, Du H, Roher A,
Yu J, Soto C, Schmidt AM, Stern D, et al. Nat
Med 2000, 6: 643-651.
8. Chavakis T, Bierhaus A, Al-Fakhri N et al. J Exp
Med 2003, 198: 1507-1515.
9. Sasaki N, Takeuchi M, Chowei H, Kikuchi S et al
Neurosci Lett 2002, 326: 117-120.
10. Chapman MR, Robinson LS, Pinkner JS et al. Scie
nce 2002, 295: 851-855
11. Bierhaus A, Humpert PM, Morcos M, et al. J Mol
Med 2005, 83: 876- 886.
12. Hofmann MA, Drury S, Fu C, Qu W et al. Cell 199
9, 97: 889-901.
13. Stern DM, Yan SD, Yan SF, and Schmidt AM. Agein
g Res Rev. 2002, 1: 1-15.
14. Hudson, B.I., Harja, E., Moser, B. and Schmidt,
A.M. Arterioscler Thromb Vasc. Biol. 2005, 25:
879?82.
15. Hanford, L.E., Enghild, J.J. et al. J. Biol. Ch
em. 2004, 279: 50019?24.