Anti-IL-18 (Human) mAb bulk_10mg

IP - 5 ug/0.5 ug recombinant Human IL-18 NT - Induction of IFN - u by KG - 1 cell (Human myelomonocyte: ATCC CCL 246) in response to the 40 ng/mL recombinant Human IL - 18 was neutralized by this antibody. The neutralization activity of lot 010 is as follows; Antibody concentration Inhibition dose* 0.1 ug/mL > 50% 1.0 ug/mL > 90% *Neutralization activity can be varied depends on cell conditions, IL - 18 concentration. ELISA - Suggested paired clone for ELISA is 159 - 12B.

Interleukin 18 (IL - 18) is a n 18 kDa cytokine which identified as a c ostimulatory factor for production of interferon - γ (IFN - γ ) in response to toxic shock and shares functional similarities with IL - 12. IL - 18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molec ule. IL - 1 converting enzyme (ICE, Caspase - 1) cleaves pro - IL - 18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL - 18 is produced from Kupffer cells, activated macroph ages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN - γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN - γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL - 18 acts on T helper type - 1 (Th1) T cells and in combination with IL - 12 strongly induces them to produce IFN - γ . Pleiotropic effects of IL - 18 has also been reported, such as, enhancement production of IFN - γ and GM - CSF in p eripheral blood mononuclear cells, production of Th1 cytokines, IL - 2, GM - CSF and IFN - γ in T cells, enhancement of Fas ligand expression by Th1 cells.

1 mg/mL

100ug IgG in 100ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 3606 Mouse: 16173

Recombinant human IL-18

This antibody reacts with human IL - 18 on Western blotting.

1 year

This antibody was purified from hybridoma (clone 125-2H) supernatant fluid using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with Balb/c mouse splenocyte immunized with recombinant human IL - 18.

IL-18