MBL

Anti-GPI-80 (Human) mAb

Product Code:
 
MBL-D087-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
3H9
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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MBL-D087-3100 ug£323.00
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Further Information

Applications:
WB - 1-5 ug/mL (chemiluminescence detection system) FCM - 1-10 ug/mL (final concentration)
Background:
The GPI-80 molecule (80 kDa) recognized by this antibody (clone 3H9) was shown to be present on human neutrophils. When 3H9 was added with a neutrophil stimulant (fMLP), the inhibition of neutrophil adherence was observed after 60 minutes incubation. 3H9 enhanced not only fMLP-induced chemotaxis but random migration of neutrophil as well. Furthermore, 3H9 clearly discriminated neutrophils from both basophils and eosinophils derived from humans.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 8875
Immunogen Translated:
Human neutrophils activated with PMA
Reactivity:
This antibody reacts with human GPI-80 on Western blotting and Flow cytometry.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 3H9) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell X63-Ag8.653 with Balb/c mouse splenocyte immunized with PMA activated human neutrophil.
Target:
GPI-80/VNN2

Documents

References

1) Sendo, D., et al., Yamagata Med. J. 23, 69-82 (2005) 2) Yoshitake, H., et al., J. Leukoc. Biol. 71, 205-211 (2002) 3) Dahlgren, C., et al. J. Leukoc. Biol. 69, 57-62 (2001) 4) Huang, J., et al. Microbiol. Immunol. 45, 467-71 (2001) 5) Nakamura-Sato, Y., et al., J. Leukoc. Biol., 68, 650-654 (2000) 6) Suzuki, K., et al. J. Immunol. 162, 4277-84 (1999) 7) Ohtake, K., et al., Microbiol. Immunol. 41, 67-72 (1997) Clone 3H9 is used in these references.