Anti-Mincle (Mouse) mAb

WB - 1-5 ug/mL (chemiluminescence detection system) IP - 5-10 ug/200 uL cell extract from 1 x 106 cells FCM - 5-10 ug/mL (final concentration) Function - 1-10 ug/mL (final concentration)

Macrophage-inducible C-type lectin (Mincle, also called as Clec4e and Clecsf9), a type ? transmembrane C-type lectin receptor, is expressed mainly in macrophages. Mincle is selectively associated with the Fc receptor common γ-chain which contains immunoreceptor tyrosine-based activation motif (ITAM) and activates macrophages to produce inflammatory cytokines and chemokines. Mincle expressing cells are activated by spliceosome associated protein 130 (SAP130) released from dead cells. Recently, Mincle is reported to recognize Mycobacterium tuberculosis as well as pathogenic fungus Malassezia species. Mincle is demonstrated to be an essential receptor for a mycobacterial glycolipid, trehalose-6,6?-dimycolate (TDM). The cytokine/chemokine production of macrophages which Malassezia or M.tuberculosis induces in Mincle-/- mice is significantly impaired. Mincle is considered to play a crucial role in immune responses to these ligands.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 26253 Mouse: 56619

RBL-2H3 cells introduced Mincle mouse full-length protein

This antibody reacts with mouse Mincle on Western blotting, Immunoprecipitation and Flowcytometry.

1 year

This antibody was purified from hybridoma (clone 4A9) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Wister rat splenocyte immunized with RBL-2H3 cells expressing full length mouse mincle.

CLEC4E