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MBL

Anti-Atg12 (Human) mAb

Product Code:
 
MBL-M154-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1 κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
6E5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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MBL-M154-3100 uL£341.00
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  • Further Information
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Further Information

Applications:
WB - 1 ug/mL (chemiluminescence detection system) IP - 5 ug/250 uL of cell extract from 1x107 cells ICC - 10 ug/mL
Background:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Gene IDs:
Human: 9140 Mouse: 67526
Immunogen Translated:
KLH-conjugated synthetic peptide (Human Atg12, internal reagion)
Reactivity:
This antibody reacts with human Atg5-Atg12 complex (55 kDa) on Western blotting. Because almost all Atg12 exist in the form of Atg5-Atg12 complex, it is difficult to detect the monomeric Atg12.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 6E5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the KLH conjugated synthetic peptide corresponding to internal region of human Atg12.
Target:
Atg12

Documents

References

1) Mizushima, N., et al., J. Cell Sci. 116, 1679-1688 (2003) 2) Mizushima, N., et al., FEBS Lett. 532, 450-454 (2002) 3) Tanida, I., et al., J. Biol. Chem. 276, 1701-1706 (2001)