Elabscience

EIF4G1 Polyclonal Antibody

Product Code:
 
E-AB-61769
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of HeLa cells using EIF4G1 Polyclonal Antibody at 1:500 dilution.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using EIF4G1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Western blot analysis of extracts of HeLa cells using EIF4G1 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EIF4G1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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E-AB-61769-60uL60uL£210.00
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E-AB-61769-120uL120uL£291.00
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E-AB-61769-200uL200uL£431.00
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Further Information

Abbreviation:
EIF4G1
Background:
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
154kDa/158kDa/166kDa/171kDa/175kDa/176kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human EIF4G1
ObservedMW:
220KDa
Purification method:
Affinity purification
Target Synonym:
EIF4G1;EIF-4G1;EIF4F;EIF4G;EIF4GI;P220;PARK18
UNIProt ID:
Q04637

Documents