Elabscience

(KO Validated) PHD2/EGLN1 Polyclonal Antibody

Product Code:
 
E-AB-90883
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines using PHD2/EGLN1 Polyclonal Antibody at 1:1000 dilution.
2 / 2
Immunohistochemistry of paraffin-embedded rat heart using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
❮❯

Western blot analysis of extracts of various cell lines using PHD2/EGLN1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat heart using [KO Validated] PHD2/EGLN1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

No additional charges, what you see is what you pay! *

CodeSizePrice
E-AB-90883-60uL60uL£224.00
Quantity:
E-AB-90883-120uL120uL£317.00
Quantity:
E-AB-90883-200uL200uL£465.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: China.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Abbreviation:
PHD2/EGLN1
Background:
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
36kDa/43kDa/46kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human PHD2/EGLN1
ObservedMW:
50KDa
Purification method:
Affinity purification
Target Synonym:
EGLN1;C1orf12;ECYT3;HALAH;HIF-PH2;HIFPH2;HPH-2;HPH2;PHD2;SM20;ZMYND6
UNIProt ID:
Q9GZT9

Documents