Elabscience

(KO Validated) Bcl-2 Polyclonal Antibody

Product Code:
 
E-AB-93314
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at 1:500 dilution.
2 / 5
Western blot analysis of extracts from wild type(WT) and Bcl-2 knockout (KO) 293T cells using Bcl-2 Polyclonal Antibody at 1:1000 dilution.
3 / 5
Immunohistochemistry of paraffin-embedded human thyroid cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 5
Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at 1:500 dilution.
Western blot analysis of extracts from wild type(WT) and Bcl-2 knockout (KO) 293T cells using Bcl-2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human thyroid cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-93314-60uL60uL£224.00
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E-AB-93314-120uL120uL£317.00
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E-AB-93314-200uL200uL£465.00
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Further Information

Abbreviation:
Bcl-2
Background:
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
22kDa/26kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human Bcl-2
ObservedMW:
26KDa
Purification method:
Affinity purification
Target Synonym:
BCL2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2
UNIProt ID:
P10415

Documents