Elabscience

Vimentin Polyclonal Antibody

Product Code:
 
E-AB-93320
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 8
Western blot analysis of extracts of HeLa cells using Vimentin Polyclonal Antibody at 1:1000 dilution.
2 / 8
Immunohistochemistry of paraffin-embedded human colon using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 8
Immunohistochemistry of paraffin-embedded human liver cancer using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 8
Immunohistochemistry of paraffin-embedded mouse brain using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 8
Immunohistochemistry of paraffin-embedded rat testis using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 8
Immunohistochemistry of paraffin-embedded rat uterus using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retri
7 / 8
Immunofluorescence analysis of NIH-3T3 cells using Vimentin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
8 / 8
Immunofluorescence analysis of U2OS cells using Vimentin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of HeLa cells using Vimentin Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat testis using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat uterus using Vimentin Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retri
Immunofluorescence analysis of NIH-3T3 cells using Vimentin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Vimentin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-93320-60uL60uL£210.00
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E-AB-93320-120uL120uL£291.00
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E-AB-93320-200uL200uL£431.00
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Further Information

Abbreviation:
Vimentin
Background:
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
53kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human Vimentin
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
VIM; CTRCT30; HEL113; vimentin
UNIProt ID:
P08670

Documents