Elabscience

TFEB Polyclonal Antibody

Product Code:
 
E-AB-93321
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 6
Western blot analysis of extracts of various cell lines using TFEB Polyclonal Antibody at 1:1000 dilution.
2 / 6
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFEB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 6
Immunohistochemistry of paraffin-embedded mouse lung using TFEB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 6
Immunohistochemistry of paraffin-embedded rat lung using TFEB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 6
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] TFEB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of U2OS cells using [KO Validated] TFEB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines using TFEB Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFEB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse lung using TFEB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using TFEB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] TFEB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using [KO Validated] TFEB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-93321-60uL60uL£210.00
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E-AB-93321-120uL120uL£291.00
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E-AB-93321-200uL200uL£431.00
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This product comes from: China.
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Further Information

Abbreviation:
TFEB
Background:
Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'; efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
53kDa/54kDa/55kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human TFEB
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
TFEB;ALPHATFEB;BHLHE35;TCFEB
UNIProt ID:
P34897

Documents