NSJ Bioreagents

PD-L1 Antibody / B7-H1 / CD274

Product Code:
 
NSJ-F55054
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
AC37
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot and store the PD-L1 antibody at 2-8oC.
1 / 5
IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC staining of FFPE human esophageal squamous carcinoma tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
4 / 5
IHC staining of FFPE human NCI-H226 cells (left) and PD-L1 transfected HEK293 cells (right) with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
5 / 5
Western blot testing of PD-L1 transfected human 293 cell lysate (left) and non-transfected 293 cell lysate (right). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
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IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human tonsil tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human esophageal squamous carcinoma tissue with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human NCI-H226 cells (left) and PD-L1 transfected HEK293 cells (right) with PD-L1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Western blot testing of PD-L1 transfected human 293 cell lysate (left) and non-transfected 293 cell lysate (right). Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).

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CodeSizePrice
NSJ-F55054-0.1ML0.1ml£502.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 1:500-1:1000,Immunohistochemistry (FFPE): 1:100-1:400
Application Note:
Optimal dilution of the PD-L1 antibody should be determined by the researcher.
Description:
As a ligand for the inhibitory receptor PDCD1/PD-1, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response. [UniProt]
Format:
Purified
Formulation:
Antibody in 1X PBS with 0.05% BSA and 0.05% ProClin 300
Limitation:
This PD-L1 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
Q9NZQ7

Documents