NSJ Bioreagents

CD79a Antibody

Product Code:
 
NSJ-V8264SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ZL7-4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD79a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Immunofluorescent staining of PFA-fixed human Raji cells with CD79a antibody (clone ZL7-4, green) and Reddot nuclear stain (red).
2 / 3
Flow cytometry testing of human Raji cells with CD79a antibody (clone ZL7-4); Red=isotype control, Blue= CD79a antibody.
3 / 3
SDS-PAGE analysis of purified, BSA-free CD79a antibody (clone ZL7-4) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human Raji cells with CD79a antibody (clone ZL7-4, green) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with CD79a antibody (clone ZL7-4); Red=isotype control, Blue= CD79a antibody.
SDS-PAGE analysis of purified, BSA-free CD79a antibody (clone ZL7-4) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8264SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
IgM complex isolated from Daudi cells was used as the immunogen for this CD79a antibody.
Limitation:
This CD79a antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P11912

Documents