NSJ Bioreagents

Moesin Antibody

Product Code:
 
NSJ-V9046SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM562
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Moesin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC: Formalin-fixed, paraffin-embedded human placenta stained with Moesin antibody (SPM562)
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IHC: Formalin-fixed, paraffin-embedded human melanoma stained with Moesin antibody (clone SPM562).
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Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone SPM562, green) and Reddot nuclear stain (red).
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Western blot testing of human PC3 lysate with Moesin antibody (clone SPM562). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.
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IHC: Formalin-fixed, paraffin-embedded human placenta stained with Moesin antibody (SPM562)
IHC: Formalin-fixed, paraffin-embedded human melanoma stained with Moesin antibody (clone SPM562).
Immunofluorescent staining of PFA-fixed human HeLa cells with Moesin antibody (clone SPM562, green) and Reddot nuclear stain (red).
Western blot testing of human PC3 lysate with Moesin antibody (clone SPM562). Predicted molecular weight ~68 kDa but routinely observed at 68-78 kDa.

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NSJ-V9046SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes 78kDa moesin protein. Moesin, a member of the Talin-4.1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for this Moesin antibody.
Limitation:
This Moesin antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P26038

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