NSJ Bioreagents

CD74 / CLIP Antibody

Product Code:
 
NSJ-V9108SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM523
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the anti-CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Flow cytometry testing of human Raji cells with anti-CD74 antibody (clone SPM523); Red=isotype control, Blue= anti-CD74 antibody.
2 / 3
IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-CD74 antibody (clone SPM523).
3 / 3
Immunofluorescent staining of human Raji cells with anti-CD74 antibody (clone SPM523, green) and Reddot nuclear stain (red).
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Flow cytometry testing of human Raji cells with anti-CD74 antibody (clone SPM523); Red=isotype control, Blue= anti-CD74 antibody.
IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-CD74 antibody (clone SPM523).
Immunofluorescent staining of human Raji cells with anti-CD74 antibody (clone SPM523, green) and Reddot nuclear stain (red).

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NSJ-V9108SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
SU-DHL-4 lymphoma cells were used as the immunogen for this anti-CD74 antibody.
Limitation:
This anti-CD74 antibody is available for research use only.
Localization:
Cell surface and paranuclear globular
Purity:
Protein G affinity chromatography
Uniprot #:
P04233

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