NSJ Bioreagents

HSP27 Antibody / HSPB1

Product Code:
 
NSJ-V9024IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM252
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
2 / 3
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
3 / 3
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone SPM252) as confirmation of integrity and purity.
❮❯

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).
SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone SPM252) as confirmation of integrity and purity.

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NSJ-V9024IHC-7ML7 ml£534.00
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Immunogen:
Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
Limitation:
This HSP27 antibody is available for research use only.
Localization:
Predominantly cytoplasmic with some nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P04792

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