NSJ Bioreagents

LC3B Antibody / MAP1LC3B (N-Terminal)

Product Code:
 
NSJ-R20312
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM293
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the recombinant LC3B antibody at -20oC.
1 / 3
ICC staining of human HeLa cells untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody (red) at 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
2 / 3
Western blot testing of lysate from HeLa cells, untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody at 1:200 dilution. Predicted molecular weight ~15 kDa.
3 / 3
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant LC3B antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
❮❯

ICC staining of human HeLa cells untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody (red) at 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
Western blot testing of lysate from HeLa cells, untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody at 1:200 dilution. Predicted molecular weight ~15 kDa.
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant LC3B antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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NSJ-R20312-0.1ML100ul£524.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100-1:200,Immunocytochemistry: 1:100-1:200,Western Blot: 1:100-1:200
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human LC3B (Autophagy-related protein LC3 B; Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the N-terminus of human LC3B was used as the immunogen for the recombinant LC3B antibody.
Limitation:
This recombinant LC3B antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q9GZQ8

Documents