NSJ Bioreagents

Integrin beta 1 Antibody / ITGB1 / CD29

Product Code:
 
NSJ-R20311
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM285
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the recombinant ITGB1 antibody at -20oC.
1 / 3
IHC testing of formalin fixed and paraffin embedded human colon tissue with recombinant ITGB1 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant ITGB1 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Western blot testing of human HeLa cell lysate with recombinant ITGB1 antibody at 1:4000 dilution. Predicted molecular weight: 88-150 kDa depending on glycosylation level.
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IHC testing of formalin fixed and paraffin embedded human colon tissue with recombinant ITGB1 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of formalin fixed and paraffin embedded human brain tissue with recombinant ITGB1 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with recombinant ITGB1 antibody at 1:4000 dilution. Predicted molecular weight: 88-150 kDa depending on glycosylation level.

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NSJ-R20311-0.1ML100ul£524.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100-1:200,Western Blot: 1:2000-1:4000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant ITGB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human Integrin beta-1 (ITGB1, CD29).
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the residues in human Integrin beta-1 was used as the immunogen for the recombinant ITGB1 antibody.
Limitation:
This recombinant ITGB1 antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P05556

Documents